顾敬敏教授

来源:动物医学学院 发稿时间:2019-06-24 浏览次数:

姓名:

顾敬敏

性别:

职称:

教授

(唐敖庆青年学者)

硕导/博导:

博导+硕导

最高学位:

博士

最高学历:

研究生

办公电话:

0431-87836406

邮箱:

jingmin0629@163.com

 

 

详细情况

学科专业:

预防兽医学

研究方向:

超级耐药细菌的噬菌体及其抗菌蛋白的生物学特性与分子作用机制、噬菌体与宿主细菌互作机制

讲授课程:

本科生:兽医免疫学、兽医微生物学、微生物学

研究生:分子病原细菌学

教育经历:

2008.092014.06,吉林大学,预防兽医学,博士,导师:韩文瑜

2004.092008.06,河北北方学院,动物医学,学士

工作经历:

2020.09 - 至今,吉林大学,动物医学学院,教授(博导)

2016.09 - 2020.09,吉林大学,动物医学学院,副教授(博导)

2014.09 - 2016.08,吉林大学,动物医学学院,讲师

科研项目:

1.   国家自然科学基金面上项目,32072824,基于三维结构和分子机制构建跨肺炎克雷伯菌K1K2荚膜型解聚酶的研究,2021/01-2024/1258万元,在研,主持

2.   吉林省自然科学基金-自然科学基金(学科布局项目),20200201120JC,噬菌体解聚酶Depo32抗肺炎克雷伯菌的作用机制,2020/01-2022/12,8万元,在研,主持

3.   国家自然科学基金,联合基金重点项目(子课题),U19A2038,饲料端禁抗后仔猪重要细菌感染的生态演替及基于抗菌肽的防治机制,2020/01-2023/1250万元,在研,子课题主持

4.   国家自然科学基金面上项目,31872505,绿色气球菌噬菌体新型裂解酶AVPL的生物学特性及其跨菌种抗葡萄球菌的分子机制,2019/01-2022/1259万元,在研,主持

5.   吉林大学优青培养计划,419080520259,重点培养阶段,2019/01-2020/12,18万元,在研,主持

6.   吉林省科技发展计划项目(优秀青年人才基金项目),20190103106JH,浅绿色气球菌噬菌体AVP的生物学特性及其治疗感染的效果评价,2019/01-2020/12,7万元,在研,主持

7.   国家重点研发计划,2017YFD0501000,“畜禽重大疫病防控与高效安全养殖综合技术研发” 重点专项“动物疫病生物防治性制剂研制与产业化”子课题“噬菌体裂解酶生物治疗制剂研制”,2017/07-2020/1245万元,在研,主持

8. 国家自然科学基金青年项目,31502103,金黄色葡萄球菌噬菌体穿孔素及其与裂解酶CHAP片段重组蛋白的构建与作用机制,2016/01-2018/1224万元,结题,主持

9. 国家自然科学基金面上项目,31572553,金黄色葡萄球菌噬菌体裂解酶LysGH15的抗原性及其代谢动力学,2016/01-2019/1269.6万元,在研,子课题主持

10. 国家自然科学基金重点项目,31130072,一种新型噬菌体及其裂解酶的分子生物学特征及作用靶位的生物结构,2012/01-2016/12277万元,结题,第一参加人

学术论文:

发表代表性文章

[1]      Song   J#, Niu W#, Wu R, Wang J, Lei L, Han W, Gu J*.   The Phage Holin HolGH15 Exhibits Potential As an Antibacterial Agent to   Control Listeria monocytogenes. Foodborne Pathog Dis. 2020 Sep 21. doi:   10.1089/fpd.2020.2833.

[2]     Wang   X#, Ji Y#, Su J#, Xue Y, Xi H, Wang Z, Bi L,   Zhao R, Zhang H, Yang L, Guo Z, Guan Y, Feng X, Sun C, Lei L, Ur Rahman S,   Dong J, Han W, Gu J*. Therapeutic Efficacy of Phage   PIZ SAE-01E2 Against Abortion Caused by Salmonella abortus equi in Mice. Appl   Environ Microbiol. 2020 Oct 28;86(22):e01366-20.

[3]     Xi   H#, He D#, Li D#, Liu S, Wang G, Ji Y, Wang   X, Wang Z, Bi L, Zhao R, Zhang H, Yang L, Guo Z, Han W and Gu J*   (2020) Bacteriophage Protects Against Aerococcus viridans Infection in a   Murine Mastitis Model. Front Vet Sci. 2020 Aug 28;7:588.

[4]     Cheng   M#, Luo M#, Xi H#, Zhao Y, Le S, Chen LK,   Tan D, Guan Y, Wang T, Han W, Wu N*, Zhu T*, Gu J*.   The characteristics and genome analysis of vB_ApiP_XC38, a novel phage   infecting Acinetobacter pittii. Virus Genes. 2020 Aug;56(4):498-507.

[5]     Yalu   Ji#, Feng Xiao#, Wei Zhu#, Shanshan Liu, Xin   Feng, Changjiang Sun, Liancheng Lei, Jianbao Dong, Ahrar Khan, Wenyu Han and Jingmin   Gu*. LysGH15 Effectively Control Murine Mastitis Caused by   Staphylococcus aureus. Pak Vet J, 40(4): 519-522.

[6]     Xue   Y#, Zhai S#, Wang Z#, Ji Y, Wang G, Wang T,   Wang X, Xi H, Cai R, Zhao R, Zhang H, Bi L, Guan Y, Guo Z*, Han W*   and Gu J*. The Yersinia Phage X1 Administered Orally   Efficiently Protects a Murine Chronic Enteritis Model Against Yersinia   enterocolitica Infection. Front. Microbiol. 2020. 11:351.

[7]     Guo   Z, Lin H, Ji X, Yan G, Lei L, Han W, Gu J*, Huang J*.   Therapeutic applications of lytic phages in human medicine. Microb Pathog.   2020 Feb 5;142:104048.

[8]     Wang   X, Xi H, Su J, Cheng M, Wang G, He D, Cai R, Wang Z, Guan Y, Sun C, Feng X,   Lei L, Ur Rahman S, Dong J*, Han W* and Gu J*.   Characterization and Genome Analysis of a Novel Escherichia coli   Bacteriophage vB_EcoS_W011D. Pak Vet J, 2020, 40(2): 157-162.

[9]     Cai   R#, Wang Z#, Wang G, Zhang H, Cheng M, Guo Z, Ji Y, Xi   H, Wang X, Xue Y, Ur Rahman S, Sun C, Feng X, Lei L, Tong Y, Han W, Gu   J*. Biological properties and genomics analysis of   vB_KpnS_GH-K3, a Klebsiella phage with a putative depolymerase-like protein.   Virus Genes. 2019 Oct;55(5):696-706.

[10] Cai   R, Wang G, Le S, Wu M, Cheng M, Guo Z, Ji Y, Xi H, Zhao C, Wang X, Xue Y,   Wang Z, Zhang H, Fu Y, Sun C, Feng X, Lei L, Yang Y, ur Rahman S, Liu X*,   Han W* and Gu J* (2019) Three Capsular   Polysaccharide Synthesis-Related Glucosyltransferases, GT-1, GT-2 and WcaJ,   Are Associated With Virulence and Phage Sensitivity of Klebsiella pneumoniae.   Front. Microbiol. 10:1189

[11] Xi   H#, Dai J#, Tong Y#, Cheng M, Zhao F, Fan H,   Li X, Cai R, Ji Y, Sun C, Feng X, Lei L, Rahman S, Han W* and Gu   J*. The characteristics and genome analysis of   vB_AviM_AVP, the first phage infecting Aerococcus viridans. Viruses.   2019 Jan 26;11(2).

[12] Ji   Y#, Cheng M#, Zhai S#, Xi H, Cai R, Wang Z,   Zhang H, Wang X, Xue Y, Li X, Sun C, Feng X, Lei L, Ur Rahman S, Han W*,   Gu J*. Preventive effect of the phage VB-SavM-JYL01   on rabbit necrotizing pneumonia caused by Staphylococcus aureus. Vet   Microbiol. 2019 Feb;229:72-80.

[13] Gu   J#,   Zhang L#, Yuan X, Chen YG, Gao X, Li D*. Synthesis and   Antibacterial Activity of Polyoxometalates with Different Structures. Bioinorg   Chem Appl. 2018 Dec 9;2018:9342326.

[14] Cai   R#, Wu M#, Zhang H, Zhang Y, Cheng M, Guo Z, Ji Y, Xi H,   Wang X, Xue Y, Sun C, Feng X, Lei L, Tong Y, Liu X, Han W*, Gu   J*. A smooth-type, phage-resistant Klebsiella pneumoniae   mutant strain reveals OmpC is indispensable for GH-K3 infection. Appl   Environ Microbiol. 2018 Oct 17;84(21).

[15] Zhang   Y#, Cheng M#, Zhang H#, Dai J, Guo Z, Li X,   Ji Y, Cai R, Xi H, Wang X, Xue Y, Sun C, Feng X, Lei L, Han W*, Gu   J*. Antibacterial effects of phage lysin LysGH15 on   planktonic cells and biofilms of diverse staphylococci. Appl Environ   Microbiol. 2018 Jul 17;84(15).

[16] Cheng   M#, Zhang L#, Zhang H, Li X, Wang Y, Xia F, Wang B, Cai   R, Guo Z, Zhang Y, Ji Y, Sun C, Feng X, Lei L, Yang Y, Han W, Gu J*.   An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for   Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin   Wounds. Viruses. 2018 May 6;10(5).

[17] Gu   J*,   Xi H, Cheng M, Han W*. Phage-derived lysins as therapeutic agents   against multidrug-resistant Enterococcus faecalis. Future Microbiol.   2018 Mar;13:275-278.

[18] Cheng   M, Zhang Y, Li X, Liang J, Hu L, Gong P, Zhang L, Cai R, Zhang H, Ge J, Ji Y,   Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*, Gu J*.   Endolysin LysEF-P10 shows potential as an alternative treatment strategy for   multidrug-resistant Enterococcus faecalis infections. Sci Rep. 2017   Aug 31;7(1):10164.

[19] Guo   Z, Huang J, Yan G, Lei L, Wang S, Yu L, Zhou L, Gao A, Feng X, Han W, Gu   J*, Yang J*. Identification and   Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia   coli Phage vB_EcoM_ECOO78. Front Microbiol. 2017 Aug 2;8:1460.

[20] Cheng   M#, Liang J#, Zhang Y, Hu L, Gong P, Cai R, Zhang L,   Zhang H, Ge J, Ji Y, Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*, Gu   J*. The Bacteriophage EF-P29 Efficiently Protects against   Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut   Microbiota Imbalance in a Murine Bacteremia Model. Front Microbiol.   2017 May 9;8:837.

[21] Zhang   L#, Li D#, Li X, Hu L, Cheng M, Xia F, Gong P, Wang B,   Ge J, Zhang H, Cai R, Wang Y, Sun C, Feng X, Lei L, Han W*, Gu   J*. LysGH15 kills Staphylococcus aureus without being   affected by the humoral immune response or inducing inflammation. Sci Rep.   2016 Jul 7;6:29344.

[22] Gu   J, Li X, Yang M, Du C, Cui Z, Gong P, Xia   F, Song J, Zhang L, Li J, Yu C, Sun C, Feng X, Lei L, Han W*.   Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic   pneumonia. Vet Microbiol. 2016 Jul 15;190:5-11.

[23] Gong   P#, Cheng M#, Li X, Jiang H, Yu C, Kahaer N, Li J,   Zhang L, Xia F, Hu L, Sun C, Feng X, Lei L, Han W, Gu J*.   Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its   endolysin LysEFm5. Virology. 2016 May;492:11-20.

[24] Song   J, Xia F, Jiang H, Li X, Hu L, Gong P, Lei L, Feng X, Sun C, Gu J*,   Han W*. Identification and characterization of HolGH15: the holin   of Staphylococcus aureus bacteriophage GH15. J Gen Virol. 2016   May;97(5):1272-81.

[25] Xia   F, Li X, Wang B, Gong P, Xiao F, Yang M, Zhang L, Song J, Hu L, Cheng M, Sun   C, Feng X, Lei L, Ouyang S, Liu ZJ, Li X, Gu J*, Han   W*. Combination Therapy of LysGH15 and Apigenin as a New Strategy   for Treating Pneumonia Caused by Staphylococcus aureus. Appl Environ   Microbiol. 2015 Oct 16;82(1):87-94

[26] Yang   M, Du C, Gong P, Xia F, Sun C, Feng X, Lei L, Song J, Zhang L, Wang B, Xiao   F, Yan X, Cui Z, Li X, Gu J*, Han W*.   Therapeutic effect of the YH6 phage in a murine hemorrhagic pneumonia model. Res   Microbiol. 2015 Oct;166(8):633-43.

[27] Gu   J, Feng Y, Feng X, Sun C, Lei L, Ding W,   Niu F, Jiao L, Yang M, Li Y, Liu X, Song J, Cui Z, Han D, Du C, Yang Y,   Ouyang S*, Liu ZJ*, Han W*. Structural and   biochemical characterization reveals LysGH15 as an unprecedented   "EF-hand-like" calcium-binding phage lysin. PLoS Pathog.   2014 May 15;10(5):e1004109.

[28] Gu   J, Liu X, Yang M, Li Y, Sun C, Lu R, Song   J, Zhang Q, Lei L, Feng X, Du C, Yu H, Yang Y, Han W*. Genomic   characterization of lytic Staphylococcus aureus phage GH15: providing new   clues to intron shift in phages. J Gen Virol. 2013 Apr;94(Pt   4):906-15.

[29] Gu   J, Liu X, Lu R, Li Y, Song J, Lei L, Sun   C, Feng X, Du C, Yu H, Yang Y, Han W*. Complete genome sequence of   Staphylococcus aureus bacteriophage GH15. J Virol. 2012   Aug;86(16):8914-5.

[30] Gu   J, Liu X, Li Y, Han W*, Lei L,   Yang Y, Zhao H, Gao Y, Song J, Lu R, Sun C, Feng X. A method for generation   phage cocktail with great therapeutic potential. PLoS One.   2012;7(3):e31698.

[31] Gu   J#,   Lu R#, Liu X, Han W*, Lei L, Gao Y, Zhao H, Li Y, Diao   Y. LysGH15B, the SH3b domain of staphylococcal phage endolysin LysGH15,   retains high affinity to staphylococci. Curr Microbiol. 2011   Dec;63(6):538-42.

[32] Gu   J, Xu W, Lei L, Huang J, Feng X, Sun C,   Du C, Zuo J, Li Y, Du T, Li L, Han W*. LysGH15, a novel   bacteriophage lysin, protects a murine bacteremia model efficiently against   lethal methicillin-resistant Staphylococcus aureus infection. J Clin   Microbiol. 2011 Jan;49(1):111-7.F1000论文)

著作教材:

1. 《噬菌体学》,科学出版社,2021,参编。

2. 《高级动物免疫学》,科学出版社,2016,参编。

3. 《免疫学原理与技术》,高等教育出版社,2016,参编。

获奖情况:

1.   2019年入选吉林大学优秀青年教师培养计划;

2.   2015年获吉林省优秀博士学位论文;

3.   2012年获教育部国务院学位委员会颁发的博士研究生学术新人奖。

社会兼职:

1.   中国微生物学会医学微生物学与免疫学专委会噬菌体学组第一届委员;

2.   兽医微生物学专业委员会的青年委员;

3.   担任Frontiers   in Cellular and InfectionMicrobiology杂志的topic编辑

4.   担任Appl   Environ MicrobiolApplied Microbiology and   BiotechnologyFuture virologyFrontier   in microbiologyFrontiers in MedicineAntibioticsVirus   ResearchArchives of VirologyScientific   ReportsJournal of Applied   MicrobiologyViruses等杂志的审稿人.