预防兽医学系

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  • 顾敬敏教授
  • 性别:男
  • 职称:教授
  • 硕导/博导:博导
  • 学科专业:预防兽医学·兽医微生物学与免疫学
  • 最高学位:博士
  • 研究方向:噬菌体与细菌互作、超级耐药细菌的治疗性噬菌体、噬菌体相关抗菌蛋白等
  • 最高学历:研究生
  • 邮箱:jingmin0629@163.com

讲授课程

本科生:微生物学

硕士生:分子病原细菌学

教育经历

2008.09–2014.06,吉林大学,预防兽医学,博士(硕博连读)

2004.09–2008.06,河北北方学院,动物医学,学士

工作经历

2020.09-至今,吉林大学,动物医学学院,教授(唐敖庆领军教授)

2016.09-2020.09,吉林大学,动物医学学院,副教授

2014.09-2016.08,吉林大学,动物医学学院,讲师

科研项目

1.国家自然科学基金优秀青年科学基金项目,32222083,重要人兽共患病原菌的噬菌体及其抗菌蛋白,2023/01-2025/12,200万元,在研,主持

2.科技部, 国家重点研发计划, 2021YFA0911201, 铜绿假单胞菌人工噬菌体高效制剂的合成与应用, 2021/10 至 2026/09, 90万元, 在研, 课题主持

3.国家自然科学基金面上项目,32072824,基于三维结构和分子机制构建跨肺炎克雷伯菌K1和K2荚膜型解聚酶的研究,2021/01-2024/12,58万元,在研,主持

4.吉林省自然科学基金(学科布局项目),20200201120JC,噬菌体解聚酶Depo32抗肺炎克雷伯菌的作用机制,2020/01-2022/12,8万元,在研,主持

5.国家自然科学基金,联合基金重点项目(子课题),U19A2038,饲料端禁抗后仔猪重要细菌感染的生态演替及基于抗菌肽的防治机制,2020/01-2023/12,50万元,在研,子课题主持

6.国家自然科学基金面上项目,31872505,绿色气球菌噬菌体新型裂解酶AVPL的生物学特性及其跨菌种抗葡萄球菌的分子机制,2019/01-2022/12,59万元,在研,主持

7.吉林大学优青培养计划,419080520259,重点培养阶段,2019/01-2020/12,18万元,在研,主持

8.吉林省科技发展计划项目(优秀青年人才基金项目),20190103106JH,浅绿色气球菌噬菌体AVP的生物学特性及其治疗感染的效果评价,2019/01-2020/12,7万元,在研,主持

9.国家重点研发计划,2017YFD0501000,“畜禽重大疫病防控与高效安全养殖综合技术研发” 重点专项“动物疫病生物防治性制剂研制与产业化”子课题“噬菌体裂解酶生物治疗制剂研制”,2017/07-2020/12,45万元,在研,主持

10.国家自然科学基金青年项目,31502103,金黄色葡萄球菌噬菌体穿孔素及其与裂解酶CHAP片段重组蛋白的构建与作用机制,2016/01-2018/12,24万元,结题,主持

11.国家自然科学基金面上项目,31572553,金黄色葡萄球菌噬菌体裂解酶LysGH15的抗原性及其代谢动力学,2016/01-2019/12,69.6万元,在研,子课题主持

12.国家自然科学基金重点项目,31130072,一种新型噬菌体及其裂解酶的分子生物学特征及作用靶位的生物结构,2012/01-2016/12,277万元,结题,第一参加人

学术论文

[1]Xi H, Ji Y, Fu Y, Chen C, Han W, Gu J*. Biological characterization of the phage lysin AVPL and its efficiency against Aerococcus viridans-induced mastitis in a murine model. Appl Environ Microbiol. 2024 Aug 21;90(8):e0046124.(2区,3.9)

[2]Zhao R, Jiang S, Ren S, Yang L, Han W, Guo Z*, Gu J*. A novel phage putative depolymerase, Depo16, has specific activity against K1 capsular-type Klebsiella pneumoniae. Appl Environ Microbiol. 2024 Apr 17;90(4):e0119723.(2区,3.9)

[3]Ji Y, Xi H, Chen C, Sun C, Feng X, Lei L, Han W, Gu J*. The pig intestinal phageome is an important reservoir and transfer vector for virulence genes. Sci Total Environ. 2024 Jan 12:170076.(1区,10.75)

[4]Ji Y, Zhao Z, Jiang Q, Loor JJ, Song L, Ou H, Liu M, Sun C, Feng X, Lei L, Han W, Li X*, Gu J*. Potential of phage EF-N13 as an alternative treatment strategy for mastitis infections caused by multidrug-resistant Enterococcus faecalis. J Dairy Sci. 2023 Aug 23:S0022-0302(23)00455-1. (1区,3.5)

[5]Ruopeng Cai#, Zhuolu Ren#, Rihong Zhao#, Yan Lu, Xinwu Wang, Zhimin Guo, Jinming Song, Wentao Xiang, Rui Du, Xiaokang Zhang, Wenyu Han, Heng Ru*, Jingmin Gu*. Structural biology and function features of phage-derived depolymerase Depo32 on Klebsiella pneumoniae with K2 serotype capsular polysaccharides. Microbiology Spectrum. 2023 Sep 26;11(5):e0530422..(1区,3.7)

[6]Ji Y, Xi H, Zhao Z, Jiang Q, Chen C, Wang X, Li F, Li N, Sun C, Feng X, Lei L, Han W*,Gu J*. Metagenomics analysis reveals potential pathways and drivers of piglet gut phage-mediated transfer of ARGs. Sci Total Environ. 2023 Feb 10;859(Pt 2):160304. (1区,10.75)

[7]Wang X#, Ji Y#, Qiu C, Zhang H, Bi L, Xi H, Lei L, Liu B, Han W,Gu J*. A phage cocktail combined with the enteric probiotic Lactobacillus reuteri ameliorated mouse colitis caused by S. typhimurium. Food Funct. 2022 Aug 15;13(16):8509-8523.(2区,6.31)

[8]Wang X#, Xing Y#, Ji Y, Xi H, Liu X, Yang L, Lei L, Han W,Gu J*.The Combination of Phages and Faecal Microbiota Transplantation Can Effectively Treat Mouse Colitis Caused by Salmonella enterica Serovar Typhimurium. Front Microbiol. 2022 Jul 7;13:944495. (2区,6.06)

[9]Wang Z, Liu X, Shi Z, Zhao R, Ji Y, Tang F, Guan Y, Feng X, Sun C, Lei L, Han W, Du XD*,Gu J*. A novel lysin Ply1228 provides efficient protection against Streptococcus suis type 2 infection in a murine bacteremia model. Vet Microbiol. 2022 Apr 4;268:109425.(1区,3.3)

[10]Liu X#, Huang C#, Qiu C, Wang Z, Cheng M, Zhang Y, Qiao Y, Guan Y, Feng X, Sun C, Li N, Li F, Lei L, Han W*, Zhang X*,Gu J*. Rapid and sensitive detection of Staphylococcus aureus using biolayer interferometry technology combined with phage lysin LysGH15. Biosens Bioelectron. 2022 Feb 15;198:113799.(1区,10.618)

[11]Song J#, Niu W#, Wu R#, Wang J, Lei L, Han W,Gu J*. The Phage Holin HolGH15 Exhibits Potential As an Antibacterial Agent to Control Listeria monocytogenes. Foodborne Pathog Dis. 2021 Aug;18(8):574-581.(3区,IF:3.171)

[12]Wang X#, Ji Y#, Su J#, Xue Y, Xi H, Wang Z, Bi L, Zhao R, Zhang H, Yang L, Guo Z, Guan Y, Feng X, Sun C, Lei L, Ur Rahman S, Dong J, Han W,Gu J*.Therapeutic Efficacy of Phage PIZ SAE-01E2 Against Abortion Caused by Salmonella abortus equi in Mice. Appl Environ Microbiol. 2020 Oct 28;86(22):e01366-20.(2区,IF:4.792)

[13]Xi H#, He D#, Li D#, Liu S, Wang G, Ji Y, Wang X, Wang Z, Bi L, Zhao R, Zhang H, Yang L, Guo Z, Han W andGu J*(2020) Bacteriophage Protects Against Aerococcus viridans Infection in a Murine Mastitis Model. Front Vet Sci. 2020 Aug 28;7:588.(2区,IF:3.412)

[14]Xue Y#, Zhai S#, Wang Z#, Ji Y, Wang G, Wang T, Wang X, Xi H, Cai R, Zhao R, Zhang H, Bi L, Guan Y, Guo Z*, Han W*andGu J*. The Yersinia Phage X1 Administered Orally Efficiently Protects a Murine Chronic Enteritis Model Against Yersinia enterocolitica Infection. Front. Microbiol. 2020. 11:351. (IF=4.235)(2区,IF:5.64)

[15]Cai R, Wang G, Le S, Wu M,Cheng M, Guo Z, Ji Y, Xi H, Zhao C,Wang X, Xue Y, Wang Z, Zhang H,Fu Y, Sun C, Feng X, Lei L, Yang Y,ur Rahman S, Liu X*, Han W*andGu J*.Three Capsular PolysaccharideSynthesis-RelatedGlucosyltransferases,GT-1, GT-2and WcaJ, Are Associated WithVirulence and Phage Sensitivityof Klebsiella pneumoniae.Front. Microbiol. 2019,10:1189 .(2区,IF:4.235)

[16]XiH#, DaiJ#, TongY#, ChengM, ZhaoF, FanH, LiX, CaiR, JiY, SunC, FengX, LeiL, RahmanS, HanW*andGuJ*.The characteristics and genome analysis of vB_AviM_AVP, the first phage infecting Aerococcus viridans.Viruses. 2019 Jan 26;11(2).(2区,IF:3.816)

[17]Ji Y#, Cheng M#, Zhai S#, Xi H, Cai R, Wang Z, Zhang H, Wang X, Xue Y, Li X, Sun C, Feng X, Lei L, Ur Rahman S, Han W*,Gu J*.Preventive effect of the phage VB-SavM-JYL01 on rabbit necrotizing pneumonia caused by Staphylococcus aureus.Vet Microbiol. 2019 Feb;229:72-80.(1区,IF:3.03)

[18]Cai R#, Wu M#, Zhang H, Zhang Y, Cheng M, Guo Z, Ji Y, Xi H, Wang X, Xue Y, Sun C, Feng X, Lei L, Tong Y, Liu X, Han W*,Gu J*.A smooth-type, phage-resistant Klebsiella pneumoniae mutant strain reveals OmpC is indispensable for GH-K3 infection.Appl Environ Microbiol. 2018 Oct 17;84(21).(2区,IF:4.016)

[19]Zhang Y#, Cheng M#, Zhang H#, Dai J, Guo Z, Li X, Ji Y, Cai R, Xi H, Wang X, Xue Y, Sun C, Feng X, Lei L, Han W*,Gu J*.Antibacterial effects of phage lysin LysGH15 on planktonic cells and biofilms of diverse staphylococci.Appl Environ Microbiol. 2018 Jul 17;84(15).(2区,IF:4.016)

[20]Cheng M#, Zhang L#, Zhang H, Li X, Wang Y, Xia F, Wang B, Cai R, Guo Z, Zhang Y, Ji Y, Sun C, Feng X, Lei L, Yang Y, Han W,Gu J*.An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds.Viruses. 2018 May 6;10(5).(2区,IF:3.811)

[21]Cheng M, Zhang Y, Li X, Liang J, Hu L, Gong P, Zhang L, Cai R, Zhang H, Ge J, Ji Y, Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*,Gu J*.EndolysinLysEF-P10 shows potential as an alternative treatment strategy for multidrug-resistant Enterococcus faecalis infections.Sci Rep. 2017 Aug 31;7(1):10164.(3区,IF:4.122)

[22]Guo Z, Huang J, Yan G, Lei L, Wang S, Yu L, Zhou L, Gao A, Feng X, Han W,Gu J*, Yang J*.Identification and Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia coli Phage vB_EcoM_ECOO78.Front Microbiol. 2017 Aug 2;8:1460.(2区,IF:4.019)

[23]Cheng M#, Liang J#, Zhang Y, Hu L, Gong P, Cai R, Zhang L, Zhang H, Ge J, Ji Y, Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*,Gu J*.The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model.Front Microbiol. 2017 May 9;8:837.(2区,IF:4.019)

[24]Zhang L#, Li D#, Li X, Hu L, Cheng M, Xia F, Gong P, Wang B, Ge J, Zhang H, Cai R, Wang Y, Sun C, Feng X, Lei L, Han W*,Gu J*.LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation.Sci Rep. 2016 Jul 7;6:29344.(2区,IF:4.259)

[25]Gu J, Li X, Yang M, Du C, Cui Z, Gong P, Xia F, Song J, Zhang L, Li J, Yu C, Sun C, Feng X, Lei L, Han W*. Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic pneumonia.Vet Microbiol. 2016 Jul 15;190:5-11.(1区,IF:2.628)

[26]Gong P#, Cheng M#, Li X, Jiang H, Yu C, Kahaer N, Li J, Zhang L, Xia F, Hu L, Sun C, Feng X, Lei L, Han W,Gu J*.Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its endolysin LysEFm5.Virology. 2016 May;492:11-20.(3区,IF:3.353)

[27]Xia F, Li X, Wang B, Gong P, Xiao F, Yang M, Zhang L, Song J, Hu L, Cheng M, Sun C, Feng X, Lei L, Ouyang S, Liu ZJ, Li X,Gu J*, Han W*.Combination Therapy of LysGH15 and Apigenin as a New Strategy for Treating Pneumonia Caused by Staphylococcus aureus.Appl Environ Microbiol. 2015 Oct 16;82(1):87-94(3区,IF:3.807)

[28]Gu J, Feng Y, Feng X, Sun C, Lei L, Ding W, Niu F, Jiao L, Yang M, Li Y, Liu X, Song J, Cui Z, Han D, Du C, Yang Y, Ouyang S*, Liu ZJ*, Han W*. Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin.PLoS Pathog. 2014 May 15;10(5):e1004109.(1区,IF:7.562)

[29]Gu J, Liu X, Yang M, Li Y, Sun C, Lu R, Song J, Zhang Q, Lei L, Feng X, Du C, Yu H, Yang Y, Han W*.Genomic characterization of lytic Staphylococcus aureus phage GH15: providing new clues to intron shift in phages.J Gen Virol. 2013 Apr;94(Pt 4):906-15.(3区,IF:3.529)

[30]Gu J, Liu X, Lu R, Li Y, Song J, Lei L, Sun C, Feng X, Du C, Yu H, Yang Y, Han W*.Complete genome sequence of Staphylococcus aureus bacteriophage GH15.J Virol. 2012 Aug;86(16):8914-5.(2区,IF:5.076)

[31]Gu J, Liu X, Li Y, Han W*, Lei L, Yang Y, Zhao H, Gao Y, Song J, Lu R, Sun C, Feng X.A method for generation phage cocktail with great therapeutic potential.PLoS One. 2012;7(3):e31698.(3区,IF:3.737)

[32]Gu J, Xu W, Lei L, Huang J, Feng X, Sun C, Du C, Zuo J, Li Y, Du T, Li L, Han W*.LysGH15, a novel bacteriophage lysin, protects a murine bacteremia model efficiently against lethal methicillin-resistant Staphylococcus aureus infection.J Clin Microbiol. 2011 Jan;49(1):111-7.(F1000论文)(2区,IF:4.153)

著作教材

1.《噬菌体学——从理论到应用》,科学出版社,2021,参编

2.《高级动物免疫学》,科学出版社,2016,参编。

3.《免疫学原理与技术》,高等教育出版社,2016,参编。

获奖情况

1.2019年获吉林大学优秀青年教师培养计划

2.2015年获吉林省优秀博士学位论文

3.2012年获教育部国务院学位委员会颁发的博士研究生学术新人奖

社会兼职

1.中国生物工程学会噬菌体学专委会委员;

2.兽医微生物学专业委员会的青年委员;

3.吉林省人兽共患病学会常务理事;

4.担任Journal of Virology、Appl Environ Microbiol、Frontier in microbiology等杂志的审稿人。

联系我们

学院电话:0431-87836150

动物医院电话:0431-87836297 或 87836298

地址:吉林省长春市西安大路5333号